Chromatography

What type of plates to choose? Support: Polyester: recommended for eluents with high water content. Withstand temperatures of 160 ° C and all solvents. Aluminium: can be used at higher temperatures than the polyester films. Use of eluents with high proportion of mineral acids or ammonia, solubilizing aluminum. Absorbent: Cellulose: partition chromatography through polar interaction. Used for the separation of polar substances (amino acids, carboxylic acids, carbohydrates ...) Silica: normal phase chromatography. Wide area of use. Fluorescent indicator: detection of substances under the UV lamp at 254 nm. Green fluorescence is eliminated at the level of the substance if it absorbs from 230 nm. For revelation of amino acids with ninhydrin, it is preferable to use plates without fluorescent indicator. Warning: acid-sensitive. Lire la suite