Yeast transgenic kit: Expression of a protein of fluorescence

Ref:117115
  • Demonstrative PW through bioluminiscence Achievable without centrifuge
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Data sheet Yeast transgenic kit: Expression of a protein of fluorescence

Description

This kit provides a new protocol that is very simplified, allowing to perform a transfer of interspecies gene. By transgenesis, the gene encoding the fluorescent protein derived from the jellyfish bioluminescent tissues will be expressed by the yeast S. cerevisiae . A very simple transformation protocol suitable for school! Jeulin designed a protocol that does not require making the cells competent. Just resuspend fresh yeast cells from a colony, mix with the transformation solution, the plasmid DNA, wave (vortex) and incubate for 30 min at 42°C. The yeasts are then cultured to verify the efficiency of the transfer, and then the revelation of the bioluminescence gene expression is performed by illuminating the boxes with UV radiation.

Content

Composition pour 15 postes :
Souche de levures sur boîte (x1)
Solution de transformation
ADN plasmidique
1 milieu prêt à couler YPD
1 milieu sélectif prêt à couler
30 Boîtes de Pétri de 55 mm,
Ensemenceur
Theme Genetics
Type of product Kits SVT